Fig. 4

AT-001 mitigates cardiac fibrosis and hypertrophy in DbCM. (a–f) Representative images and ventricular fibrotic area (%) of Masson’s Trichrome staining (a, b), representative images and ventricular fibrotic area (%) of Picro-sirius red staining (c, d), and representative images and cardiomyocyte’s cross-sectional area of wheat germ agglutinin (WGA) staining (e, f) of C57BL/6J mice with experimental DbCM (n = 5) at 3 weeks post-treatment with either vehicle control (VC) or AT-001 (40 mg/kg/day). (g, h) Protein expression of MMP9 (g), MMP7 (h) in myocardial extracts from C57BL/6J mice with experimental DbCM (n = 4) at 3 weeks post-treatment. Values represent means ± SEM. Differences were determined using an unpaired two-tailed Student’s t-test. *P < 0.05 significantly different from vehicle control-treated mice