Fig. 2

Effect of various lipid species on glucose stimulated insulin secretion in mouse islets. Mouse islets were incubated in Krebs buffer with 0 mmol/l glucose for 2 h and then treated with Krebs buffer containing 2 mmol/l glucose, 20 mmol/l glucose or 20 mmol/l glucose with increasing doses of LPC O-16:0, LPC O-18:0, DG 18:2_22:6, TG 12:0_18:2_22:6 or palmitic acid for 30 min (n = 9 in each experimental group with 10 islets per well). Insulin levels are shown as the fold change relative to the insulin secreted at 20 mmol/l glucose. Note: vehicles (ethanol or DMSO) used in the GSIS did not affect insulin secretion. ^###p < 0.001 for 2 mmol/l glucose group vs. 20 mmol/l glucose only group; *p < 0.05, **p < 0.01 and ***p < 0.001 for test groups vs. 20 mmol/l glucose only group