Fig. 3From: Neuregulin-4 attenuates diabetic cardiomyopathy by regulating autophagy via the AMPK/mTOR signalling pathway Nrg4 upregulates the suppressed autophagy in type 1 DM, and 3-MA attenuates Nrg4-upregulated autophagy. a, b Western blot and quantitative analysis of autophagy-related proteins (Beclin1, LC3-II/I and P62) in vivo. c Representative TEM images depicting autophagosomes in vitro, with arrows indicating autophagosomes. d, e Western blot and quantitative analysis of autophagy-related proteins in step 2 of the animal experiment. Data are presented as the mean ± SD (n = 6 per group). Significant differences are depicted with *P < 0.05Back to article page