Fig. 4
From: MLKL-mediated necroptosis is a target for cardiac protection in mouse models of type-1 diabetes
Effects of RIPK3 knockout in streptozocin (STZ)-induced diabetic hearts. RIPK3 knockout (RIPK3-KO) and wild-type mice (WT) were rendered diabetic by STZ injection. a The levels of phosphorylated MLKL (p-MLKL) were analyzed by western blot in the heart. Upper panel: Upper panel: a representative western blot from 2 out of 6 different hearts in each group for p-MLKL and total MLKL; Bottom panel: quantification of p-MLKL relative to total MLKL. b Necrotic cell death in the heart was determined by Evans blue staining assay. Upper panel: representative micro-photograph for necrotic cells in the heart; Bottom panel: quantification of necrotic cell death. c and d Echocardiographic analysis for myocardial function. e Myocardial fibrosis was assessed by collagen deposition in the heart. Upper panel: representative micro-photograph for collagen deposition in the heart (red); Bottom panel: quantification of collagen deposition. f and g Biomarkers of myocardial injury: serum CK-MB (f) and cardiac troponin I (g). Data are mean ± SD, n = 5–6. *P < 0.05 versus Sham + WT and †P < 0.05 versus STZ + WT (ANOVA)