Fig. 4

AZD5069 improves the concentration of circulating triglycerides, trends toward improved circulating ALT/AST, and improved platelet number to spleen cross-sectional area ratio (PSR) in mice under 16 weeks of high fat diet. AZD5069 restored circulating plasma triglyceride concentrations those in mice in the CN diet arm (A), while circulating adiponectin (B) and leptin (C) concentrations were not different in mice in the HF and HF + AZD diets. Circulating liver enzymes ALT (D) and AST (E) in mice in the HF + AZD diet arms trended towards a concentration intermediate between that in mice on the CN and HF diets (p = 0.0541 HF vs. HF + AZD). PSR was reduced in in mice in the HF and HF + AZD diet arms compared to mice in the CN arm (F). Liver and Kidney clearance of BUN was significantly reduced in mice in the HF + AZD diet arm compared to mice in the CN diet arm (G), but these values were well-within the normal reference range in mice (8.0 to 33.0 mg/dL). The bars in each graph show the median of each of the measurements among n = 6–12 mice in each of the diet arms and the error bars show the SD. Statistical analysis was conducted by one-way ANOVA or Kruskal–Wallis test followed by Bonferroni post-test as appropriate. Significant differences in the medians among the diet arms are depicted with *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001