Fig. 3

AZD5069 improves insulin sensitivity under high fat diet conditions, as measured by the hyperinsulinemic-euglycermic clamp at 16 weeks. Circulating insulin levels trended lower in mice in the HF + AZD diet arm but were not significantly different. Mouse blood glucose levels during the clamp procedure. The symbols indicate the mean while the error bars indicate the SEM. Glucose infusion rates were significantly higher in mice in the HF + AZD diet arm compared to mice in the HF despite a trend of reduced level of plasma insulin. Endogenous glucose production is unaltered in mice among the diet arms until the clamp is started. Glucose flux (Rd) is unaltered in mice among the diet arms until the clamp is started. Significantly greater 2-DG uptake in skeletal muscle and brown adipose tissue in mice in the HF + AZD arm compared to mice in the other diet arms. PG AT refers to the peri-gonadal white adipose tissue and SubQ AT refers to the resected subcutaneous total fat. The bars in graphs A, D–F and the symbols in graphs B, C show the median of each of the measurements among n = 6–12 mice in each of the diet arms and the error bars show the SD. Statistical analysis was performed with a Student’s t-test or repeated measures two-way ANOVA as appropriate. Significant differences are depicted with “*” were p < 0.05