Fig. 1

Schematic representation of the study. CD34⁺ HSPCs were isolated via Mac-sorting from cord blood and characterized for GLP-1R transcript and protein expression levels, and downstream signaling pathway activation. Then, CD34⁺ HSPCs were expanded in HG condition with or without LIRA (A). In a different experimental setting the cells were treated with LIRA only after the loss of glucose tolerance (B). CD34⁺ HSPCs cultured in NG (30 mM mannitol) condition were used as control (A, B). Proliferation, migration and oxidative stress were finally assessed at the end of experiments. GLP-1R = glucagone-like peptide 1 receptor; HG = high glucose; LIRA = liraglutide; NG = normal glucose