Fig. 5

Dietary change leading to weight loss counteracts T2D-induced atrophy of PV+ interneurons. a Average soma volume of PV+ interneurons in striatum after sham surgery. b Average soma volume of PV+ interneurons in contralateral striatum and peri-infarct ipsilateral striatum after tMCAO. Data are presented as mean ± SD. Brown–Forsythe and Welch ANOVA (a) and Two-way ANOVA (b) followed by two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli was used for analyses. * Denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.001. c Representative images of PV+ interneurons in the ipsilateral striatum of non-T2D, T2D/Ob and T2D/WL mice after tMCAO. White dotted lines indicate the infarct region (infarct region is on right side of the dotted line; peri-infarct region is on left side of the dotted line). Scale bar for lower magnification images: 200 µm. Scale bar for higher magnification image inserts: 50 µm. Group sizes: a non-T2D n = 5, T2D/Ob n = 4, T2D/WL n = 4. b Non-T2D n = 7, T2D/Ob n = 6, T2D/WL n = 6