Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12933-021-01322-6/MediaObjects/12933_2021_1322_Fig5_HTML.png)
TGF-β1 and Smad3 immuno-histological staining. a Representative photograph of immunoprecipitation of anti-TGF-β1 antibody in control cardiac muscle specimen. b Representative photograph of immunoprecipitation of anti-TGF- β1 antibody in empagliflozin treated rat's cardiac muscle specimen. c Representative photograph of immunoprecipitation of anti-Smad3 antibody in control cardiac muscle specimen. d Representative photograph of immunoprecipitation of anti-Smad3 antibody in empagliflozin treated rat's cardiac muscle specimen. e Expression of TGF-β1. f. Expression of Smad3. Individual measurements (empty symbols) and average (filled symbols, n=6 and 8 to the control and empagliflozin, respectively), control (red) and empagliflozin (blue). * P < 0.05 vs. control, by student's t-test. Photographs magnification x 200.