Fig. 2

miR-181b reduces the inflammation-induced expression of tissue factor in HMEC-1. HMEC-1 were transfected with a control mimic or miR-181b as well as a negative control inhibitor or anti-miR-181b. The cells were left untreated or stimulated with TNFα for 2 h for mRNA analysis of asTF (a, b) and flTF (c, d) or 6 h for TF protein (e). VCAM1 mRNA expression (f, g) and protein (h) in HMEC-1 transfected with miR-181b or anti-miR-181b following TNFα for 2 h and 6 h, respectively. Nuclear extracts (i, left panel) and cytoplasmatic extracts (i, right panel) of HMEC transfected with miR-181b or a control mimic at rest or stimulated with TNFα for 1 h subjected to western blot analysis using antibodies against NFκB, histone H3 (H3), or β-actin. n ≥ 3, groups were compared by 2-way ANOVA test with global p-values for treatment with TNFα and Sidak’s multiple comparison post hoc test