Fig. 5

a Representative examples of mitochondrial structure in LA cardiomyocytes (dye: MitoTracker after thresholding) and b related data of mitochondrial density. c Representative example of mitochondrial structure of LA cardiomyocytes after permeabilization of the sarcolemma (above), mitochondrial Ca²⁺ during perfusion with internal solution containing either 0 µM (center) or 2 µM Ca²⁺ (below). d Related data of mitochondrial Ca²⁺ uptake, e mitochondrial density and f correlation of mitochondrial Ca²⁺ uptake and mitochondrial swelling. g Serum concentration of β-hydroxybutyrate (BHB). Statistical analysis: Two-way ANOVA followed by post-hoc Bonferroni (b, e, g) or unpaired, two-tailed Student’s t-test (d). p-values: ¹0.004, ²< 0.001, ³< 0.001, ⁴< 0.001, ⁵0.026, ⁶0.049. a–f n = cells derived from 6 animals per group. g n = animals