Fig. 5

NHE expression and effect of NHE inhibition on SA-induced inflammation in MAC. NHE 1, 5, 6 expression from total protein extracts of PLT and MAC was assessed by western blotting (a). For each different antibody a positive control (cell extract of mouse brain, kidney and liver) was used, as indicated in the datasheets. Due to technical limitation in PLT protein extract the positive control cannot be shown, simultaneously. Gene expression of pro-inflammatory cytokine/chemokine was assessed in MAC pre-treated with empagliflozin and/or amiloride both 100 µM (b) or cariporide 10 µM (c) for 16 h followed by 3 h of stearate 100 µM incubation. (Empa empagliflozin; IL interleukin, TNFα tumor necrosis factor-α, MCP monocyte chemoattractant protein, SA stearate). Gene expression data are expressed as mean ± SEM from at least 3 independent experiments (*p < 0.05, **p < 0.01 vs SA; ^¥p < 0.05 ^§p < 0.01 vs control)