Fig. 8
a Hematoxylin and eosin stained sections of myocardium illustrating cardiomyocytes in cross-section (×400). Scale bars are 20 µm. b Bar graph showing quantitative analysis of cross -sectional area of cardiomyocytes. c The effects of LIRA treatment on expression of ANP in left ventricle. c Protein expression levels of ANP detected by Western blotting. β-actin was used as an internal control. Bands are representative of two separate experiments. Relative density values of ANP, data are shown as the mean ± SEM, N = 5–6 rats per group. The significance of group differences was determined by ANOVA for factors of genotype (G), drug treatment (T) and their interaction term (I)