Fig. 5

Sacubitril/valsartan reduces a tubulointerstitial and b periarterial fibrosis in ZO rats. Representative PSR stained images show fibrosis in the renal interstitium in a or surrounding an arteriole in b. Accompanying bar graphs show quantitative analysis of average intensity of PSR staining in a and area of fibrosis normalized to arteriole diameter in b. Data are represented by mean ± SE. n = 5–7 rats per group. Symbols: *p < 0.05 versus ZLC; ^†p < 0.05 versus ZOC; ^§p < 0.05 versus ZOH. Scale bars = 50 μm. c–e Bar graphs show Col1α1 and fibronectin mRNA expression normalized to 18S mRNA and expressed as a fold change from baseline in ZLC group (n = 4 per group). *p < 0.05 versus ZLC; ^†p < 0.05 versus ZOC; ^Δp < 0.05 versus ZOSV; ^§p < 0.05 vs ZOH