Fig. 2

CR alters cellular metabolism and gene expression regulation. Representative Western blot for ERK1/2 phosphorylation and densitometry analysis of ERK1/2 normalized to GAPDH indicating attenuation in ERK overactivation following CR. n = 4 in each group, *p < 0.05 vs. WT, ^&p < 0.02 vs. db/db + AT group (a). Representative Western blot for PPARγ and densitometry analysis of PPARγ normalized to β actin showing reduction in PPARγ protein levels following CR. n = 4 in each group, *p < 0.03 vs. WT, ^#p = 0.02 vs. db/db, ^&p < 0.001 vs. db/db + AT group (b). ADIPONECTIN mRNA levels were measured in the cardiac tissue (c). SIRT (d) HDAC1/2 (e) activity were measure in the nuclear fraction from WT and db/db mice hearts. Cardiac protein levels of pAMPK relative to total AMPK western blot and densitometry (f). *p < 0.05 vs. WT, ^#p < 0.05 vs. db/db, ^&p < 0.05 vs. db/db + AT group, n = 4 in each group. Values represent mean ± SD