Fig. 6

Differential contribution of IR isoforms to VSMCs apoptosis. a Effect of thapsigargin (100 nmol/L, 18 h) on cleaved caspase 3 levels analyzed by Western blot in IRA and IRB VSMCs. b Effect of IGF-IR inhibition by PPP (1 µmol/L) on cleaved caspase 3 levels in IRB and IRLoxP^+/+ VSMCs treated with thapsigargin (100 nmol/L) for 18 h. Experiments were performed 4 times. *p < 0.05, **p < 0.005, ***p < 0.0005 vs. each control; ^††p < 0.005 vs. IRA VSMCs; ^§p < 0.05 vs. each thapsigargin; ^Δp < 0.05 vs. IRLoxP^+/+ VSMCs. PPP: picropodophyllin. c % of early apoptotic cells in G2. These cells were stained positively for Annexin V-FITC and negatively for propidium iodide. *p < 0.05 vs. each control; ***p < 0.001 vs. each control; ^†p < 0.05 vs. each thapsigargin; ^†††p < 0.001 vs. each thapsigargin; ^ΔΔΔp < 0.001 vs. each point of IRLoxP^+/+ VSMCs