Fig. 5

Antiapoptotic effect of IGF-IR on VSMCs. a Effect of IGF-IR inhibition by PPP (1 µmol/L) on cleaved caspase 3 levels analyzed by Western blot in IRLoxP^+/+ and IR^−/− VSMCs stimulated with IGF-I (10 nmol/L) or IGF-II (10 nmol/L) for 24 h. b Dose–response effect of thapsigargin (0-100 nmol/L, 18 h) on cleaved caspase 3 levels in IRLoxP^+/+ and IR^−/− VSMCs. c Effect of IGF-I or IGF-I pretreatment (10 nmol/L, 1 h) in IRLoxP^+/+ and IR^−/− VSMCs treated with thapsigargin (100 nmol/L) for 18 h. Experiments were performed 4 or 5 times. *p < 0.05, **p < 0.005, ***p < 0.0005 vs. each control; ^†p < 0.05 vs. IRLoxP^+/+ VSMCs. PPP picropodophyllin