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Fig. 1 | Cardiovascular Diabetology

Fig. 1

From: Potential role of insulin receptor isoforms and IGF receptors in plaque instability of human and experimental atherosclerosis

Fig. 1

IR isoforms expression and association with IGF-IR or IRS-1 in human carotid atherosclerotic plaques. Representative gels (a) and quantifications (b–e) of IRA (left panels) and IRB (right panels) protein levels and their association with IGF-IR, IRS-1 or p-IRS-1 (Ser307) in non-complicated regions (n = 10) and their respective complicated plaques (n = 10). 150 µg of protein from each human plaque were immunoprecipitated with IRB isoform antibody. Supernatants were subsequently immunoprecipitated with IRβ (IRA and IRB isoforms) antibody. Immune complexes from first (only IRB) or second (only IRA) immunoprecipitations were collected on protein A-agarose beads and submitted to SDS-PAGE. IRLoxP+/+ VSMCs expressing both IR isoforms were used as an immunoprecipitation control. *p < 0.05, **p < 0.005 vs. NCP. CP complicated region of atherosclerotic plaque, IP immunoprecipitation, NCP non-complicated region

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Cardiovascular Diabetology

ISSN: 1475-2840