Fig. 2
From: Liraglutide dictates macrophage phenotype in apolipoprotein E null mice during early atherosclerosis
Polarization of THP-1 monocytes to macrophages. THP-1 monocytes were differentiated into MΦ0 macrophages with 320 nmol/l PMA and further polarized to MΦ1 (100 ng/ml LPS and 20 ng/ml IFN-gamma) and MΦ2 (20 ng/ml IL-4 and 20 ng/ml IL-13) macrophages. Polarization was confirmed by analysing cell supernatants for a TNF-alpha b IL-1beta and c IL-10. Cells were analysed for d TNF-alpha and e mannose receptor (MR) by RT-qPCR and f MR by Western blotting. Error bars are representative of three independent experiments (n = 3) each carried out with two replicates. Statistical analysis was carried out performing a Friedman test followed by Dunn’s multiple comparison post-test. *p < 0.05, **p < 0.01 and ***p < 0.001 were considered statistically significant. p > 0.05 was considered NS. Stars above the columns represent comparisons made against monocyte controls while capped lines indicate comparisons made between other groups. A representative Western blot is shown with beta-actin used as a loading control