Fig. 5From: High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetesHDL mitigates the inhibition of Akt phosphorylation by dextrose. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min (a) or HDL (5–120 µg/mL) (b), *p < 0.05, **p < 0.01 relative to dextrose alone (5.7 mM). Incubation of HDL (80 µg/mL) with increasing dextrose (5.7, 20 and 40 mM), *p < 0.05, **p < 0.01 compared to relative dextrose concentration alone (c). HDL (80 µg/mL) with either dextrose (20 mM) and/or pAkt inhibitor (LY294002) for 1 h (d). Akt phosphorylation was determined by Western blot analysis. Data is expressed as a ratio of p-Akt to total-Akt. *p < 0.05 relative to the LY294002 treatment. Data shown are representative of results from 3 independent experimentsBack to article page