Fig. 4

HDL attenuates dextrose-induced inhibition of p38 activation. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min (a) or HDL (5–120 µg/mL) (b), *p < 0.05, relative to dextrose alone (5.7 mM). Incubation of HDL (80 µg/mL) with increasing dextrose (5.7, 20 and 40 mM), *p < 0.05, **p < 0.01 compared to relative dextrose concentration alone (c). HDL (80 µg/mL) with either dextrose (20 mM) and/or p38 inhibitor (SB203580) for 1 h (d). p38 phosphorylation was determined by Western blot analysis. Data is expressed as a ratio of p-p38 to total-p38. *p < 0.05 relative to the SB203580 treatment. Background media was 2.5% FBS MCDB. Data shown are representative of results from 3 independent experiments