Fig. 2

HDL attenuates dextrose-induced inhibition of ERK activation. HUVECs were incubated with increasing dextrose concentrations (5.7–60 mM) for 15 min (a), or HDL (5–120 µg/mL) (b), *p < 0.05, **p < 0.01 relative to dextrose alone (5.7 mM). Incubation of HDL (80 µg/mL) with increasing dextrose (5.7, 20 and 40 mM) in a background media of 2.5% MCDB, *p < 0.05, **p < 0.01 compared to relative dextrose concentration alone (c). HDL (80 µg/mL) with either dextrose (20 mM) and/or ERK inhibitor (PD98059) for 1 h (d). ERK phosphorylation was determined by Western blot analysis. Data is expressed as a ratio of p-ERK to total-ERK. *p < 0.05 relative to the PD98059 treatment. Data shown are representative of results from 3 independent experiments