Fig. 3

Effect of OxLDL, LDL and HDL on OCN surface expression in THP-1 cells. a OCN + THP1 cells were measured by flow cytometry after treatment with 40 μg/ml OxLDL ± 200 μg/ml HDL at different time points (n = 4 in each group). Three-way ANOVA for multiple comparisons: p < 0.001. Kruskal–Wallis test for pairwise comparison: *p < 0.05 vs untreated; ^#p < 0.05 vs OxLDL treated. b OCN + THP1 cells were measured by flow cytometry after treatment with 40 μg/ml OxLDL and different concentrations of HDL for 48 h (n = 5 in each group). Two-way ANOVA for multiple comparisons: *p < 0.001. c OCN + THP1 cells were measured by flow cytometry after 48 h treatment with 40 μg/ml OxLDL ± 200 μg/ml HDL ± 40μg/ml LDL (n = 3 in each group). Three-way ANOVA for multiple comparison: p < 0.001. *p values at the Kruskal–Wallis test for pairwise comparisons