Fig. 1

AGE, but not high glucose and high mannitol, induced BDNF release from platelets. Each platelet sample (1 × 10⁸ cells/tube) was incubated with Ca²⁺-free tyrode buffer containing 0.1% BSA (C, control), 25 mM glucose (G), 25 mM mannitol (M) and 100 μg/ml AGE (A) for 5 min at 37 °C (n = 8). Data are presented as the ratio of released BDNF/(released BDNF + BDNF content) (mean ± SEM). Statistical analysis was performed by one-way ANOVA. *p < 0.05 vs. control