Fig. 1

Schematic representation of experimental protocol. Male C57 mice were fed with HFD for 4 weeks and then injected with streptozotocin (STZ) at the dose of 90 mg/kg intraperitoneally. One week after STZ injection, mice were considered to have developed diabetes only if their 12-h fasting blood glucose levels were higher than 11.1 mmol/L. The diabetic mice were subjected to 30 min ischemia followed by 3 h reperfusion (for determination of mitochondrial dynamics, mitochondrial function, myocardial apoptosis and ROS) or 24 h reperfusion (for determination of infarct size and cardiac function). Mdivi-1 (1.2 mg/kg) or vehicle (dimethyl sulfoxide) was administrated intraperitoneally 15 min before the onset of myocardial reperfusion