Fig. 4

GRK2 protein levels increase in the hearts of long-term HFD-fed animals. a GRK2 protein levels in cardiac tissue normalized by GAPDH levels are expressed as fold-increase over SD-fed WT animals (N = 3–4 mice per genotype and condition). A representative blot is shown. The arrow indicates the band corresponding to GRK2 (as determined in the last lane using 0.5 ng of recombinant purified protein) and the asterisk an unspecific band. b mRNA levels of GRK2 were quantified by pPCR and normalized by a geometrical mean of HPRT and RPS29 (N = 5–6). Data are mean ± SEM and statistical analysis performed by two-tail unpaired Student’s t test.*P < 0.05, **P < 0.01