Fig. 2
![Fig. 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12933-016-0460-z/MediaObjects/12933_2016_460_Fig2_HTML.gif)
Effects of N-acetylcysteine treatment on the levels of 15-F2t-IsoP, NO, nitrotyrosine and O2 − in diabetic myocardium, as well as NO and O2 − levels in isolated cardiomyocytes. Control (C) or STZ-induced diabetic rats were either untreated (D) or treated with antioxidant N-acetylcysteine (1.5 g/kg/day, NAC) by oral gavage for 4 weeks,and adult rats cardiomyocytes were isolated and prepared. a–e In situ O2 − production in LV sections stained by DHE (a), myocardial levels of 15-F2t-IsoP (b), NO (c), nitrotyrosine (d) and O2 − in the absence and presence of L-NAME (e); f, g cardiomyocytes levels of NO (f) and O2 − (g) production in the absence and presence of L-NAME. All the results are expressed as mean ± SD, n = 7. Differences of O2 − in the absence and presence of L-NAME were determined by using two-way repeated-measures ANOVA followed by Bonferroni’s post hoc test, the others were determined by using one-way ANOVA followed by Tukey’s test. *P < 0.05 vs. all the other groups. MLU, mean light unit