Fig. 7
From: Interleukin-1β mediates high glucose induced phenotypic transition in human aortic endothelial cells
Anti-IL-1β antibodies treatment inhibited high glucose-induced phenotypic transition of HAECs, asassessed by laser scanning confocal microscopy. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow heads). c The administration of anti-IL-1β antibodies treatment caused a reduction of these changes (P < 0.05). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a high glucose concentration (30 mM) + anti-IL-1β antibodies (1000 ng/ml) treatment for 48 h. Experiments were repeated three times. Scale bar, 75 μm.*P < 0.05 vs.HG