Upregulation of inducible NO synthase by exogenous adenosine in vascular smooth muscle cells activated by inflammatory stimuli in experimental diabetes

Table 1 Effects of exogenous adenosine/AMP on cytokine-induced iNOS synthesis in aortic smooth muscle cells

	Relative densitometric analysis of iNOS levels (arbitrary units)
	Control (n = 7)	Diabetes (n = 8)
No addition	100 ± 8	100 ± 12
Adenosine 1 µM	95 ± 12	91 ± 7
Adenosine 10 µM	90 ± 12	114 ± 7
Adenosine 100 µM	97 ± 9	161 ± 14*
Adenosine 100 µM + EHNA 1 µM	112 ± 10	152 ± 14*
Adenosine 100 µM + NBTI 10 µM	93 ± 10	154 ± 22*
Adenosine 100 µM + MRS1706 1 µM^a	90 ± 15	108 ± 11^**
AMP 100 µM	108 ± 6	150 ± 12*
AMP 100 µM + AOPCP 200 µM	115 ± 9	176 ± 19*

Rat aortic smooth muscle cells from control and diabetic rats were incubated with 10 ng/mL interleukin (IL)-1β, 10 ng/mL interferon (IFN)-γ and 25 ng/mL tumor necrosis factor (TNF)-α plus 1 µg/mL LPS for 24 h. iNOS was detected by Western blot. Densitometric analysis of protein level is expressed as % of No addition
EHNA erythro-9-(2-Hydroxy-3-nonyl)adenine, NBTI S-(4-Nitrobenzyl)-6-thioinosine, AOPCP α,β-Methylene-ADP
* P < 0.01 vs. No addition, ** P < 0.05 vs. Adenosine 100 µM (two-tailed t test)
^a n = 3

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