Fig. 4

The effect of high ambient glucose on corin and ANP expression in neonatal rat cardiomyocytes and H9c2 cardiomyoblasts. a, b Quantification for corin (a) and ANP (b) mRNA levels in neonatal rat cardiomyocytes was measured by real-time qPCR, neonatal rat cardiomyocytes were treated with normal or high glucose for 36 h (n = 6 for each group in a, n = 5 for each group in b). c–e The corin and ANP protein levels of neonatal rat cardiomyocytes at 36 h were measured by western blot (n = 6 for each group in d, n = 5 for each group in e). f–h Corin and ANP expression in neonatal rat cardiomyocytes at different culture time (n = 4 for each group in g, n = 5 for each group in h). i Immunofluorescence analysis of corin expression in neonatal rat cardiomyocytes at 36 h was performed by confocal fluorescence microscope. Bar 40 μm. j Corin expression in H9c2 cardiomyoblasts at 36 h was detected by real-time qPCR (n = 6 for each group). k Corin and ANP expression in H9c2 cardiomyoblasts at different culture time was determined by western blot. l Immunofluorescence analysis of corin in H9c2 cardiomyoblasts at 36 h was performed by confocal fluorescence microscope. Bar 80 μm. Ctrl: normal glucose, 5.5 mM glucose; OC: osmotic control, 5.5 mM glucose plus 19.5 mM mannitol; HG: high glucose, 25 mM glucose. Data are presented as mean ± SD. *P < 0.05 and **P < 0.01 versus Ctrl