Fig. 2
From: Short-term high glucose exposure impairs insulin signaling in endothelial cells
Insulin 100 pmol/L slightly increased phospho-Ser1177eNOS at 5 and 10 min, with its major effect occurring at 30 min. HUVECs were treated with 100 pmol/L insulin at different time points: 5, 10 and 30 min. Whole cell lysates were prepared for Western Blot analysis. The panels show representative images of different independent experiments. Densitometric values were normalized to total eNOS. Bars represent mean ± SEM for five independent experiments. *P < 0.05 NG with insulin 100 pmol/L vs. NG w/o insulin treatment