Figure 1

Animals were subject to a 2 h euglycemic clamp of 3 mU/kg/min insulin or saline infusion with or without AICAR. Arterial blood samples (droplets) were taken for glucose and lactate analysis. A bolus injection (i.v.) of AICAR (20 mg/kg) is indicated by pointing hand and followed by AICAR infusion (3.75 mg/min/kg). Other bolus infusions are indicated by pointing hand. In protocol A, arterial and venous blood samples were taken (indicated as A–V) for plasma glucose determination as well as arterial insulin and AICAR determination. The gastrocnemius group of muscles was freeze clamped at 120 min for the determination of AICAR and ZMP content as well as R’g determination. In protocol B, microbubble infusion (40 µL/min) and periods where ultrasound measurement of MvV and MvP were made are shown by CEU. Muscle contraction (field stimulation, 2 Hz, 0.1 ms, 30–50 V) is indicated by EX. In protocol C L-NAME (NOS inhibitor) was infused 15 min prior to and during AICAR infusion.