Fig. 1

Characterization of cardiovascular lines. a Western blot analysis against AgT and p53 to check the immortalization performed in neonatal cardiomyocyte lines. β-actin was used as charge control. b Characterization of neonatal cardiomyocytes with a specific marker as TnT by Western blot or by immunofluorescence. Characterization of ECs with specific markers as PECAM-1 or CD31 by flow cytometry (c) or vWF by immunofluorescence (d). Western blot analysis of insulin signalling in CMs (e, f) and in ECs (g). *p < 0.05 vs. control