Figure 3

TRPV1 promotes lipolysis of 3T3-L1 preadipocytes through the regulation of Cx43-mediated intracellular calcium levels. A. Images indicate the Fluorescence Recovery After Photobleaching (FRAP), after treatment with capsaicin (Cap), capsaicin + capsazepine (Capz) or capsaicin + 18α-GA (18α-GA) for 24 h, used to measure the dynamics of Cx43 mobility. B The ratio of fluorescence recovery inside the bleached regions. *P < 0.05 vs Con, ^#P < 0.05 vs Cap. The values were expressed as the mean ± SEM from 3 separate experiments. C. Immunoblots of TRPV1, p-CaM, Cx43, CaMKII, PPARδ, and HSL in 3T3-L1 preadipocytes treated with free fatty acids (FFA), FFA + capsaicin (Cap), FFA + capsaicin + capsazepine (Capz), FFA + capsaicin + EGTA (EGTA), FFA + capsaicin + 18α-GA (18α-GA) or FFA + capsaicin + GSK0660 (GSK0660, PPARδ inhibitor, 10 μmol/L) for 24 h. *P < 0.05, **P < 0.01 vs Con; ^#P < 0.05, ^##P < 0.01vs FFA; ^ΔP < 0.05, ^ΔΔP < 0.01 vs Cap. The densitometric values of protein expression levels were all normalized to GAPDH. Values are expressed as the mean ± SEM; n = 3 per group.