Figure 3

Upregulation of PKC leads to decreased PDGFR-α expression in hyperglycemic endothelial cells. A: Western blot analysis of PKC-α in HUVECs treated with 5.5 mM (Low) or 30 mM (High) glucose. B: Relative quantification data of Western blot analysis of PKC-α, PKC-βII, and PKC-δ in HUVECs treated with 5.5 mM (Low) or 30 mM (High) glucose. **P < 0.01 vs Low glucose (n = 4 for each group). Data represent means ± standard error of the mean. C: HUVECs exposed to 5.5 mM (Low) or 30 mM (High) glucose were treated with or without bisindolylmaleimide I (Bis I) at a concentration of 4 μΜ for 30 minutes and then were subject to Western blot analysis for PKC-α and PDGFR-α expression. D: Relative expression levels of PKC-α and PDGFR-α in the experiment of Figure 3C. *P < 0.05, **P < 0.01 vs Low glucose, Bis I (−) group. #P < 0.05, ##P < 0.01 vs High glucose, Bis I (−) group. n = 4 for each group. Data represent means ± standard error of the mean.