Immunohistochemical staining in the abdominal aorta of control (Ctr) and high-fat/cholesterol atherogenic diet minipigs (HFC). (A) Immunohistochemical staining for inflammatory cytokines in the abdominal aorta, NF-ĸB p65: nuclear factor ĸB p65; IL-1β: interleukin 1 beta; TNF-α: tumor necrosis factor alpha; MCP-1: monocyte chemoattractant protein-1; VCAM-1: vascular cell adhesion molecule 1; ICAM-1: intercellular adhesion molecule 1; MMP-9: matrix metalloproteinase 9; CRP: C-reactive protein; left, 40×; and right, 400×. (B) NF-ĸB p65, IL-1β, TNF-α, MCP-1, VCAM-1, ICAM-1, MMP-9, and CRP protein levels were quantified in HFC and Ctr samples. Data are presented as mean ± SEM. a, P < 0.05 and b, P < 0.01 versus the Ctr group.