Figure 2

Effects of GLAP on MCP-1 (A), ICAM-1 (B), VCAM-1 (C), and PAI-1 gene expression in HUVECs (D). HUVECs were treated with or without 10 μg/ml GLAP in the presence or absence of 5 μg/ml RAGE-Ab for 4 hr. Then total RNAs were extracted, transcribed and amplified by real-time PCR. Data were normalized by the intensity of 18S mRNA-derived signals and then related to the value obtained with control. # and ##, p < 0.05 and p < 0.01 compared to the value with 10 μg/ml GLAP alone, respectively. N = 3-6 per group.