Figure 3

Mechanisms involved in the endothelium impairment by IL-1β. Effects of (A) anakinra (AK, 10, 50, and 100 μg/mL), (B) apocynin (Apo; 10 μmol/L), (C) tempol (100 μmol/L), (D) indomethacin (Indo; 10 μmol/L), or (E) 1400 W (10 μmol/L) on the impairment produced by 2.5 ng/mL IL-1β on the endothelium-dependent relaxations induced by ACh (0.1 nmol/L to 10 μmol/L) in isolated mesenteric arteries from control SD rats. Data are expressed (means ± SE) as the percentage of the previous contraction induced with 1 μmol/L NA. The number of segments used for every curve, which were obtained from 3 to 14 animals, as well as the statistical significance are in parenthesis.