| |
nLDL
|
ox-LDL
|
AGE-LDL
|
|---|
|
TBA (nmoles HMF/mg protein)
|
1.92 ± 1.30
|
4.41 ± 1.22*
|
7.35 ± 0.83**#
|
|
TNBSA (AU)
|
0.45 ± 0.08
|
0.40 ± 0.07*
|
0.32 ± 0.04*
|
|
TBARS (nmoles MDA/mg protein)
|
0.30 ± 0.21
|
4.17 ± 0.06**
|
1.82 ± 0.31*#
|
|
Rf (cm)
|
0.51 ± 0.05
|
0.63 ± 0.08*
|
0.74 ± 0.05*
|
- *indicates p < 0.05 vs. nLDL, **indicates p < 0.01 vs. nLDL and #indicates p < 0.05 vs. ox-LDL; nLDL: native low density lipoprotein; TBA: thiobarbituric assay; HMF: 5-hydroxymethylfurfural; TNBSA: trinitrobenzene sulphonic acid assay; TBARS: thiobarbituric acid reactive substances; MDA: malondialdehyde; Rf: relative electrophoretic mobility by agarose gel electrophoresis.
