GW3965 inhibited diabetes-induced myocardial apoptosis. a. Representative image of TUNEL immunostaining. Left panel: 20 ×μmagnification, scale bar 100 μm. Right panel: TUNEL stain of box of left panel; 40 ×μmagnification, scale bar 25 μm. TUNEL positive cells were brown. b. The percentage of TUNEL positive cells was calculated. c-d. Myocardial apoptosis was determined by Western blot analysis of cleaved caspase-3 (n =5-6) and quantification of caspase-3 activation (n =6-10). *P < 0.05 or **P < 0.01 vs db/+ group; #P < 0.05 or ##P < 0.01 vs db/db group. Abbreviations: GW, GW3965; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; cas-3, caspase-3.