Figure 1
From: p38 mitogen-activated protein kinase is involved in arginase-II-mediated eNOS-Uncoupling in Obesity
HFD feeding enhances eNOS, Arg-II expression/activity, and p38mapk activation in mouse aortas. (A) Immunoblotting analysis of eNOS total protein and eNOS-S1177 (p-eNOS) levels (n = 5). (B) Immunoblotting analysis of Arg-II (Arg-I not detectable, tubulin served as loading control), p38mapk activation, i.e., Thr180/Tyr182-phosphorylated p38mapk (p-p38mapk) and total p38mapk in the aortas of WT mice fed NC or HFD (n = 5), and arginase activity assay (n = 7-9). *p < 0.05 vs NC. In case of analyzing total protein levels such as Arg-II and eNOS, the ratio of Arg-II/tubulin or eNOS/tubulin in NC group serves as reference. The levels of eNOS and Arg-II expression in HFD group are calculated as fold changes to those of the NC group.