Role of UCP-2 in the aorta from Control and BATIRKO mice under high-fat diet. (A) qRT-PCR analysis of UCP-2 expression levels in aorta from Control and BATIRKO mice under HFD. (B) Representative photomicrographs of OilredO staining of aortic roots from Control and BATIRKO under high-fat diet and quantification of lipid content and its correlation with UCP-2 expression levels in the aorta. (C) Determination of TNF-α plasma levels (by ELISA) and in WAT and aorta (by qRT-PCR). (D) Spearman correlation between UCP-2 and TNF-α levels in the aorta by qRT-PCR. (E) Plasma insulin levels by ELISA in Control and BATIRKO under high-fat diet. (F) Spearman correlation between UCP-2 expression levels in aorta and plasma insulin levels. Control under HFD (Control; n = 12); moderate hyperinsulinemic obese BATIRKO mice (BATIRKO MH, n = 6); normoinsulinemic obese BATIRKO mice (BATIRKO N, n = 10). *p < 0.05 vs. Control; †p < 0.05 vs. BATIRKO MH mice.