Role of UCP-2 in the aorta from Control and ApoE−/−mice. (A) Representative photomicrographs of OilredO staining of aortic roots and quantification of lesion area and lipid content from Control and ApoE−/− mice at 8, 12, 18 or 24 weeks of age. (B) qRT-PCR analysis of UCP-2 mRNA expression in aorta artery. (C) Spearman correlation between UCP-2 expression levels in the aorta by qRT-PCR and lesion area or lipid depot. Control at 8 weeks (Control 8wk, n = 3); ApoE−/− at 8 weeks (ApoE−/− 8wk, n = 3); Control at 12 weeks (Control 12wk, n = 5); ApoE−/− at 12 weeks (Control 12wk, n = 4); Control at 18 weeks (Control 18wk, n = 4); ApoE−/− at 18 weeks (ApoE−/− 18wk, n = 6); Control at 24 weeks (Control 24wk, n = 5); ApoE−/− at 24 weeks (ApoE−/− 24wk, n = 5). *p < 0.05 vs. each control; †p < 0.05 vs. ApoE−/− 8wk; §p < 0.05 vs. ApoE−/− 12wk; #p < 0.05 vs. ApoE−/− 18wk.