Figure 8

Glibenclamide inhibited Nalp3 and Caspase-1 expression by high glucose and LPS stimulation in primary peritoneal macrophages. Nalp3 and Caspase-1 in macrophages were measured by western blotting. Macrophages were incubated for 24 h under high glucose (33 mM), or high glucose + LPS (1 μg/ml), or high glucose + LPS + glibenclamide (100 μM). Cells incubated without drug treatment served as control. High glucose stimulation significantly increased Caspase-1 expression. Nalp3 and Caspase-1 levels were markedly increased by high glucose + LPS challenge. Caspase-1 level was further increased by high glucose + LPS compared with LPS group. Both proteins were significantly inhibited by glibenclamide treatment. *P < 0.05, **P < 0.01 vs control; ^#P < 0.05, ^##P < 0.01 vs Glucose + LPS; ^††P < 0.01 vs LPS. Values are means ± SEM (n = 3 per group).