Figure 4

Accumulation of macrophages in adipose tissue is inhibited by PPARgamma activation. B) Analysis of MCP-1 mRNA expression levels and B) analysis of F4/80 in abdominal fat from HFD-fed and vehicle-, rosiglitazone-, or telmisartan-treated mice after 10-weeks of intervention;°°p < 0.01 rosiglitazone-treated mice vs. vehicle-treated animals;°p < 0.05 rosiglitazone-treated mice vs. vehicle-treated animals, ** p < 0.01 telmisartan-treated mice vs. vehicle-treated animals. C) Immunohistological analysis of the gonadal fat tissue sections isolated from HFD-fed mice treated with vehicle, rosiglitazone, or telmisartan after 10-weeks of intervention; stained with monoclonal antibody detecting mouse macrophage marker (RM0029-11H3-specific antibody), as described in Methods. Positive cells are indicated by an arrow. As positive control spleen sections were used. For the negative control the same sections of gonadal adipose tissue isolated from HFD-fed mice were with unspecific IgG-antibodies, as indicated.