Figure 3

Ligand-mediated PPARgamma activation inhibits T-lymphocytes infiltration into in adipose tissue during HFD-induced IR. A) Analysis of SDF-1alpha mRNA expression levels and B) analysis of CD3gamma in abdominal fat from HFD-fed and vehicle-, rosiglitazone-, or telmisartan-treated mice after 5- and 10-weeks of intervention;°°p < 0.01 rosiglitazone-treated mice vs. vehicle-treated animals;°p < 0.05 rosiglitazone-treated mice vs. vehicle-treated animals, ** p < 0.01 telmisartan-treated mice vs. vehicle-treated animals; * p < 0.05 telmisartan-treated mice vs. vehicle-treated animals. C) Immunohistological analysis of the gonadal fat tissue sections isolated from HFD-fed and vehicle-, rosiglitazone-, or telmisartan-treated mice after 5- and 10-weeks of intervention; stained with CD3-specific antibody, as described in Methods. Positive cells are indicated by an arrow. For control staining spleen sections were used. For the negative control the same sections of gonadal adipose tissue isolated from HFD-fed mice were stained with unspecific IgG-antibodies, as indicated.