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Figure 5 | Cardiovascular Diabetology

Figure 5

From: Sirtuin1-p53, forkhead box O3a, p38 and post-infarct cardiac remodeling in the spontaneously diabetic Goto-Kakizaki rat

Figure 5

Decreased Akt phosphorylation in diabetic GK rat myocardium and increased forkhead class O 3a (FOXO3a) DNA binding 12 weeks after MI. Panel (A) shows representative western blots of myocardial tissue probed with anti-PTEN, anti-Akt, anti--phospho-Akt, anti-FOXO3a, anti-phospho-FOXO3a and anti-Tubulin as loading control. In (B) PTEN protein is overexpressed in GK+MI rats, (C) shows a decrease in the phosphorylated Akt/total Akt protein expression status in GK+MI rats compared to W+MI. In (D) phosphorylated FOXO3a/total FOXO3a protein expression status is shown. Panel (E) shows an increase in DNA--bound, myocardial FOXO3a transcription factor in the post-MI GK rats as assessed by electrophoretic mobility shift assay (EMSA). Data is presented as means ± SEM; * indicates P < 0.05 vs. W (sham-operated Wistar), # indicates P < 0.05 vs. GK (sham-operated GK), † indicates P < 0.05 vs. W+MI.

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Cardiovascular Diabetology

ISSN: 1475-2840