Effect of tempol (anti-oxidant) on Ins2+/-: Western blots showing expression of NOX-4, thioredoxin (Trx), MMP-2,-9 and TIMP-1,-3,-4 in the heart tissue of WT, Ins2+/- and Ins2+/- treated with tempol (Akita/Tempol). (i) The expression of NOX-4 increased and Trx decreased in Akita as compared to WT. However, it was attenuated after treatment with tempol. The expression of MMP-9 and TIMP-3 was increased, while MMP-2 and TIMP-4 was decreased in Akita. The treatment with tempol ameliorated the expression of MMP-2,-9 and TIMP-3,-4. There was no change in the expression of TIMP-1. Beta-actin was used as a loading control. (ii) Bar graph was based on the scanned arbitrary unit of the bands and represents the mean ± SD of six animals. *, p < 0.05 to WT; #, p < 0.05 to Akita.