A: Measurement and comparison of blood glucose level: Blood glucose level was determined at four and twenty four weeks in WT and Ins2+/- that showed robust hyperglycemia at twenty four week Akita. B: Determination and comparison of plasma homocysteine (Hcy) level: Plasma Hcy level was determined by high performance liquid chromatography (HPLC) at four and twenty four weeks in male WT and Ins2+/-. There was significant elevation in Hcy level at twenty four week in Akita. C: Echocardiogram and end diastolic diameter (EDD): Echocardiography was performed at four and twenty four weeks in male WT and Ins2+/- using SONO 5500 device. The diastolic diameter is shown by double headed arrow. It was increased at twenty four week in Akita. The end diastolic diameter (EDD) was significantly increased at twenty four week in Akita. Each bar represents average +SD of ten animals. *, p < 0.05 compared to WT.