Figure 3

Western blot analysis of the effect of HG on the synthesis of FSP1 and α-SMA protein in HAECs. A: HAECs were grown in a culture medium that contained 5.5-30 mM glucose for 48 h. Mannitol was used as a control for hyperosmolarity. B: HAECs were incubated with HG (30 mM) for 0, 12, 24, and 48 h. (0 h: 0.04 ± 0.001, 12 h: 0.65 ± 0.04, 24 h: 0.98 ± 0.04, and 48 h: 1.22 ± 0.02; ^#P < 0.05 vs. the control). C: Western blot analysis of the effect of HG on the synthesis of α-SMA and FSP1 protein in HAECs. Values are means ± SD; ^#P < 0.01 vs. NG and * P < 0.05 vs. HG. NG: normal glucose. HG: high glucose. HI+Irb: high glucose + Irbesartan. Experiments were repeated three times. Irb: Irbesartan